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Next generation of microbiological risk assessment: Potential of omics data for exposure assessment

In food safety and public health risk evaluations, microbiological exposure assessment plays a central role as it provides an estimation of both the likelihood and the level of the microbial hazard in a specified consumer portion of food and takes microbial behaviour into account. While until now mostly phenotypic…
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Authors: W.den Besten Heidy M., Alejandro Amézquita, Sara Bover-Cid, Stéphane Dagnas, Mariem Ellouze, Sandrine Guillou, George Nychas, Cian O’Mahony, Fernando Pérez-Rodriguez, Jeanne-Marie Membré
Publication date: 20/12/2018
frontiers in microbiology journal Flow Cytometric and 16S Sequencing Methodologies

Flow Cytometric and 16S Sequencing Methodologies for Monitoring the Physiological Status of the Microbiome in Powdered Infant Formula Production

The aim of this study was to develop appropriate protocols for flow cytometric (FCM) and 16S rDNA sequencing investigation of the microbiome in a powdered infant formula (PIF) production facility. Twenty swabs were collected from each of the three care zones of a PIF production facility and used for…
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Authors: Anvarian Amir H. P., Yu Cao, Shabarinath Srikumar, Séamus Fanning and Kieran Jordan
Publication date: 22/06/2016

16S rRNA Based Profiling of Bacterial Communities Colonizing Bakery-Production Environments

Conventional culture-based techniques are largely inadequate in elucidating the microbiota contained in an environment, due to low recovery within a complex bacterial community. This limitation has been mitigated by the use of next-generation sequencing (NGS)-based approaches thereby facilitating the identification and classification of both culturable and uncultivable microorganisms. Amplicon…
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Authors: Dechamma Mundanda Muthappa, Sakshi Lamba, Sathesh K. Sivasankaran, Ankita Naithani, Noel Rogers, Shabarinath Srikumar, Guerrino Macori, Amalia G.M. Scannell, and Séamus Fanning
Publication date: 11/07/2022
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